Question 256420

In an enzyme reaction experiment, the concentration of the substrate S

S is varied and the velocity measured for each concentration, giving the following data.

[S](mM)= 0.2, 0.4, 0.6, 0.8, 1.0

V(μM/min)= 0.38, 0.62, 0.79, 0.91, 1

By sketching the Lineweaver-Burk plot of 1/V against 1/[S] determine an approximation of

Km and Vmax. No need to sketch.

Which of the following is the best approximation of Km and the maximum velocity Vmax?