In an enzyme reaction experiment, the concentration of the substrate S
S is varied and the velocity measured for each concentration, giving the following data.
[S](mM)= 0.2, 0.4, 0.6, 0.8, 1.0
V(μM/min)= 0.38, 0.62, 0.79, 0.91, 1
By sketching the Lineweaver-Burk plot of 1/V against 1/[S] determine an approximation of
Km and Vmax. No need to sketch.
Which of the following is the best approximation of Km and the maximum velocity Vmax?