A sample containing mixture of methionine, proline, cysteine, histidine, and glutamic acid was subjected to electrophoresis at pH 4. Show the migration of each amino acid in the electrophoresis matrix by drawing spots corresponding to the location of each amino acid. Label each the spots with the 3-letter abbreviation of the amino acid. Show the calculation of pI of each amino acid below. The ionization of each amino acid is no longer necessary to be illustrated.
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